Biochemistry Faculty
Michael K. Chan
| |
Professor and Interim Department Chair
(joint appointment with the Department of Chemistry)
Phone: 614-292-8375
Fax: 614-292-6773
email: chan@chemistsry.ohio-state.edu
Webpage: Chan Homepage |
Research Interests:
The research of my laboratory is directed towards the study of metal
based catalysts and sensors. These are interdisciplinary areas that
require the use a wide variety of techniques from both chemical and
biochemical disciplines.
The focus of the biochemistry group is directed towards understanding
the molecular mechanisms of metalloenzymes. Utilizing macromolecular
crystallography, we obtain three-dimensional pictures of metalloproteins,
and based on these structures, we form hypotheses for how they work.
My group is actively involved in several crystallographic studies, including
peptide deformylase, a potential target for antibacterial chemotherapy;
FixL, a heme based biological oxygen sensor; and Ni-CODH, an unusual
biological enzyme capable of fundamental transformations of carbon monoxide.
The research of the chemistry group emphasizes the design of functional
synthetic systems which parallel the interests of the biological group.
Here, projects involve the design and synthesis of new ligands, preparation
of their associated metal complexes, and characterization of their physical
and chemical properties. Current targets within the group are directed
towards the design of heme analogs for asymmetric catalysis and chemical
sensors.
Publications
Selected publications from the last 5 years:
Chan SI, Wang VC, Lai JC, Yu SS, Chen PP, Chen KH, Chen CL, Chan, M. K. (2007) "Redox Potentiometry Studies of Particulate Methane Monooxygenase: Support for a Trinuclear Copper Cluster Active Site."
Angew Chem Int Ed Engl. 46(12), 1992-1994.
Isaza CE, Silaghi-Dumitrescu R, Iyer RB, Kurtz DM Jr, Chan, M. K. (2006) "Structural basis for O2 sensing by the hemerythrin-like domain of a bacterial chemotaxis protein: substrate tunnel and fluxional N terminus." Biochemistry. 45(30), 9023-31.
Jain R, Hao B, Liu RP, Chan, M. K. (2006) "Structures of E.
coli peptide deformylase bound to formate: insight into the preference
for Fe2+ over Zn2+ as the active site metal.", J Am Chem
Soc. 127(13), 4558-9.
Hao B, Zhao G, Kang PT, Soares JA, Ferguson TK, Gallucci J, Krzycki
JA, Chan, M. K. (2004) " Reactivity and chemical synthesis of L-pyrrolysine-
the 22(nd) genetically encoded amino acid." Chem Biol. 11(9), 1317-24.
Blight SK, Larue RC, Mahapatra A, Longstaff DG, Chang E, Zhao G, Kang
PT, Green-Church KB, Chan, M. K, Krzycki JA. (2004) " Direct charging
of tRNA(CUA) with pyrrolysine in vitro and in vivo." Nature
431(7006), 333-5.
Fekner T, Gallucci J, Chan, M. K. (2004) "Synthesis and metalation
of a chiral, pyridine-strapped, cyclic bis(benzimidazole) ligand.", Org
Lett. 6(6), 989-92.
Fekner T, Gallucci J, Chan, M. K. (2004) "Ruffling-induced chirality:
synthesis, metalation, and optical resolution of highly nonplanar, cyclic,
benzimidazole-based ligands." J Am Chem Soc.126(1), 223-36.
Fekner T, Gallucci J, Chan, M. K. (2003) "Intramolecular aromatic
nucleophilic substitution of the benzimidazole-activated nitro group.", Org
Lett. 5(25), 4795-8.
Jain, R. and Chan, M. K. (2003) “Mechanisms of ligand discrimination
by heme proteins”, J. Bioinorg. Chem. 8, 1-11.
Arndt, J.W., Hao, B., Ramakrishnan, V., Chen, T., Chan, S. I., and
Chan, M. K. (2002) “Structure of the hyperthermophilic Pyrococcus furiosus
carboxypeptidase”, Structure 10, 215-224.
Hao, B., Isaza, C., Arndt, J., Soltis, M., and Chan,
M. K. (2002). "Structural
analysis of the mechanism of oxygen sensing by the FixL heme domain
of Bradyrhizobium japonicum", Biochemistry 41, 12952-12958.
Hao, B., Gong, W., Ferguson, T.K., James, C.M., Krzycki, J.A., and
Chan, M. K. (2002) “Identification of novel UAG encoded residue: structure
of a methanogen methyltransferase”, Science 296, 1462-1466.
Payra, P., S.C. Hung, W.H. Kwok, D. Johnston, J. Gallucci, Chan,
M. K (2001). "Structural Magnetic and Catalytic Properties of a Self-Recognized
– Oxo-Bridged Diiron (III) Bis (benzimidazole) Complex”, Inorg.
Chem. 40, 4036-4039.
Arndt, J.W., W. Gong, X. Zhong, J Liu, Z. Lin, A.K. Schowalter, C.
Paxson, M.D. Tsai, Chan, M. K (2001). “Insight into the Catalytic Mechanism
of DNA Polymerase B: Structures of Intermediate Complexes”, Biochemistry
40, 5368-5375.
Chan, M. K. “Recent advances in heme-protein biosensors” (2001). Curr.
Opin Chem. Biol. 5, 216-222.
